promoter from P. pastoris for constitutive expression of the L protein. e original kb P PGK 1 sequence described by de Almeida et al. [ ]wasreducedtoaminimal bp sequence a er deletion analysis with restriction enzymes []. e resulting P PGK 1 sequence was used to generate the pPGK expression vector for intracellular expression. Additionally, a pPGK XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. ... RC pGPD-2 from pBR322 & GPD promoter & PGK terminator & TRP1 gene RC pMA230 ...
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  • Jun 09, 2018 · Its 5' to 3' sequence is the same as in the database (ACCESSION AE000141). (b) Presented so that the promoter sequence numbers increase from left to right. Its 5' to 3' sequence is comparable to the consensus sequence. 16. lac promoter- lactose- (or IPTG- isopropylthiogalactoside) inducible Problems with lac promoter- 1. Weak 2.
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  • pLEX-MCS PGK promoter Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). ...
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  • PGK-DTa Probe NheI Hind III Hind III 11.5 kb Hind III PGK-DTa 8.2 kb Site III Site IV Probe SphI SphI 12 kb Probe Bgl II Bgl II SphI SphI PGK-DTa Bgl II Fig. S1 Probe EcoRI PGK Neo GFP PGK Puro VMC PGK Puro K14-Agouti oeN KGP Puro
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  • The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression.
Subsequent pop-out of the promoter-URA3 would result in only one copy of the desired promoter replacing the HIS3 promoter . In order to simplify the experimental procedure, we designed upstream and downstream primers each containing a 39-base sequence identical to the target chromosome locus followed by a 20-base sequence identical to the ... compares favorably with the SV40 promoter. Although CpG islands are characteristically unmethylated (Bird 1986), and the PGK-1 island on the Xa is unmethylated, the Xi is methylated at 119 of 121 CpG dinucleotides analyzed in this region (Pfeifer et al. 1990a, b). Our earlier
The dual promoter phiC31 vectors are available in two formats: The pFC-EF1-MCS- PGK-RFP-Puro vector (cat# FC550A-1-SBI) has EF1a driving your gene´s expression cloned into the MCS and PGK expressing the RFP-Puro combo markers. The actual sequence of the clone may differ due to genetic variation and alternative splicing. The actual sequence for any of the ORF cDNA clones can be requested at any time which can be sent by e-mail. However, please note that the actual sequence for clones in pre-production or clones offered via Custom Gene Synthesis may not yet be available.
pHAGE-PGK-MCS-IRES-ZsGreen-W Description: Lentiviral vector that expresses insert from internal PGK promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; ampicillin resistance; restriction enzyme cloning. PGK-Neo-bpA loxA Contains the neomycin resistance gene expressed from the PGK promoter with the bovine growth hormone poly A site and flanked by lox P sites for easy removal. The vector used is pBluescript II KS (-). Directionality is from the T3 to T7 promoter (A).
I introduced the CAG promoter from the pCXN2 vector to the PacI site of the STOP-EFGP-ROSA TV, between SA and the floxed Neo-STOP cassette. 5. R-A4 From the Stuart Orkin lab at the Children’s Hospital, Boston. This is not a targeting vector. Instead, it is a plasmid containing a 4kb genomic DNA from the Rosa 26 locus. Dec 10, 2019 · If your sequence of interest is expected to just enhance transcription, you may need to use a minimal promoter vector to achieve a basal signal above background. If your sequence of interest is a promoter region and has all the necessary components for basal transcription, the promoterless vector will likely be suitable.
Oct 15, 2005 · LMO2 is transcribed from 2 alternate promoters leading to 2 mRNA isoforms that differ in their 5′ untranslated region (5′UTR) but encode the same protein. 25 A proximal promoter is present in the vicinity of exon 3 while a distal promoter is located nearly 25 kb upstream. 25,26 Activity of the distal promoter appears to be mostly restricted ... The vector harbors PGK promoter controlling expression of a protein of interest that is linked with RFP through IRES sequence. The PGK promoter provides robust expression in various human and mouse cell types with expression levels close to physiological.
A second SCW injection strongly reactivated Saa3-promoter activity at 1 (23.3±6.8-fold over PGK) and 3 days (40.3±8.7-fold over PGK) after challenge. To assess whether these results are specific for the Saa3 -promoter, we performed the same experiment using a Cxcl1 -promoter reporter.
  • Lifx offlineSequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniRef. Sequence clusters. Proteomes. Protein sets from fully sequenced genomes. Annotation systems. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) ARBA (System generated rules ...
  • Rocket league trade up systemSequence Details Sequence The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
  • Makeup drawer dividersThe targeting vector contains, from 5' to 3', a partial Cartpt sequence spanning exon 1 through intron 2, an FRT3 site within Cartpt intron 2, a partial Cartpt exon 3 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre ...
  • Oculus dash exclamation markJan 23, 2007 · Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniRef. Sequence clusters. Proteomes. Protein sets from fully sequenced genomes. Annotation systems. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) ARBA (System generated rules ...
  • Molloy college final exam schedule fall 2020The analysis of transformed strains in which single copies of lacZ fusions have been targeted to the qutE locus has identified three constitutive positively acting sequence elements in the pgk gene. Firstly, sequence located between -161 and -120bp (possibly spanning -120) relative to the transcript start site is essential for expression, thus demonstrating that the putative core promoter, including potential TATA and CCAAT boxes, and a pyrimidine rich region, is not alone sufficient to ...
  • Masters in software engineering without bachelors redditThe targeting vector containes, from 5' to 3', a partial Slc17a8 sequence spanning intron 11, an frt3 site within Slc17a8 intron 11, a partial Slc17a8 exon 12 sequence up to and including the endogenous stop codon, an IRES2 sequence, a Cre recombinase, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene ...
  • Minecraft coordinates resource packApr 10, 2018 · Promoter strengths under stressful conditions. To investigate the impact of stressful conditions on the responses of promoters, S. cerevisiae BY4741-derivative strains with various reporter plasmids for all the promoters were subjected to different stress conditions, including high temperature (39 °C), or in the presence of acetic acid (3.6 g/L) or furfural (1.0 g/L), respectively.
  • Rustic decorating ideas for living roomsA hybrid yeast promoter comprising constituents of the 5' non-coding PGK region and, as an upstream activation sequence, the upstream activitation sequence of the GAL10 gene of Saccharomyces cerevisiae, and which does not contain the endogenous PGK upstream activation sequence.
  • Florida hunter safety course militaryThe prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. The promoter of the mouse phosphoglucokinase gene (PGK) is used as the eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin / neomycin expression.
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The PGK promoter has been inserted between the ClaI and BamHI sites and contains sites downstream to allow a second gene to be inserted. The BsgI and BseRI restriction sites in the first MCS cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. Sequence

A novel hybrid promoter (termed P 350) was created by combining segments of two K. lactis promoters, namely, the strong constitutive P GAP1 promoter and the carbon source-sensitive P ICL1 promoter. We demonstrate that P 350 is tightly repressed in the presence of glucose or glycerol and becomes derepressed upon depletion of these compounds by ... In step1, a cassette containing neomycin phosphotransferase coding sequence (Neo, green) that is expressed from a phosphoglycerate kinase-1 (pgk) promoter (light blue) flanked by loxP elements (red) is targeted to a chosen locus, in this case Hnf3 α (yellow), by homologous recombination.